Microbial Fermentation Custom Development

BOC Sciences provides standard microbial fermentation CDMO services. More than 20 years of microbial drug research and development experience allows us to undertake everything from simple projects to the most challenging microbial fermentation projects. As your preferred partner, BOC Sciences' comprehensive services cover process/analytical development, clinical and commercial supply GMP production of various biomolecules and product types. We are good at using microorganisms cultured on the medium to produce various organic molecules with complex structures, including structures such as chiral and stereoselectivity. Our microbial fermentation services can bring better scalability and lower production cost.

Microbial Fermentation Engineering

Fermentation engineering is one of the important biotechnology, which is widely used in medicine, light industry, food, agriculture, environmental protection and other fields; while microorganisms are the core of fermentation technology. Microbial fermentation is the conversion of raw materials into desired target products through a certain metabolic method. Nowadays, with the development of genetic recombination technology, microorganisms can also be "customized" production on demand. Factors affecting the preparation of active compounds by microbial fermentation mainly include preparation of culture medium, screening of strains, fermentation temperature, fermentation time, seed age, etc. The key technology is the selection of strains. Differences in bacterial species will directly lead to differences in the types of extracellular proteases, and different cut points of proteins will directly affect the biological activity of the metabolites produced. Commonly used strains for microbial fermentation include Bacillus subtilis, Aspergillus niger, etc. The source of microbial strains is relatively wide, the protease produced does not need to be separated, and the production cost is lower than that of enzymatic hydrolysis.

Our Microbial Fermentation Services

BOC Sciences can provide integrated fermentation services for custom APIs, peptides, carbohydrates, lipids, oligonucleotides, nucleic acids, organic acids, and more. Our services include but are not limited to the following types:

• Development and optimization of strains (viruses, fungi or bacteria).
• Medium and process development, to provide a suitable medium for the subsequent fermentation process, reduce by-products, and maintain consistent quality.
• Integrate fermentation process and manufacturing, and determine the cGMP manufacturing route from early stage to clinical and commercial supply of drugs.
• Fermentation and scale-up, where fermentation methods include batch culture fermentation, fed-batch fermentation and continuous fermentation; scale-up includes semi-industrial-scale fermentation and large-scale batch fermentation.
• Downstream processing, such as centrifugation, filtration, and chromatography.

Our Microbial Fermentation Abilities

• 4 state-of-the-art cGMP factories, 3500L, 12000L and 26000L disposable reactor production lines, which can match the scale of downstream processing and meet customer needs.
• Standardized engineering bacteria construction laboratory can complete a series of projects such as high expression strain screening; stability research; shake flask culture of transformed bacteria, induced expression and other projects.
• Strict and comprehensive biosafety measures, large-scale biosafety level 2 (BSL2-LS) closed facilities and special operating procedures; highly trained operators.

What Can We Offer?

BOC Sciences offers a broad range of microbial fermentation products and solutions to the pharmaceutical, biotechnology and food industries. With an experienced team of scientists and state-of-the-art facilities, we are able to provide high-quality, cost-effective fermentation services for the production of small molecules, peptides, carbohydrates, lipids, oligonucleotides, nucleic acids, organic acids, and more.

Small Molecule APIs

At BOC Sciences, we offer comprehensive microbial fermentation services, including the production of small molecules such as terpenoids, flavonoids and phenols. Terpenoids are a class of organic compounds found in plants and animals that possess a wide range of biological activities, including anti-inflammatory, antioxidant, and antiviral properties. Flavonoids are a group of plant compounds that have been shown to have a variety of health benefits, including anti-inflammatory, antioxidant, and anti-cancer properties. Phenolics are a class of compounds found in plants that have been shown to have antioxidant and anti-inflammatory properties.

Peptides

In addition to small molecules, we also offer microbial fermentation services for the production of peptides, which are short-chain amino acids with a wide range of biological activities, including antibacterial, antiviral and anti-inflammatory properties. Peptides have a variety of applications, including pharmaceuticals, cosmetics, and food and beverage products.

Carbohydrates

Carbohydrates are another class of compounds that can be produced through microbial fermentation. We provide fermentation services for the production of polysaccharides, complex carbohydrates found in plants that have a wide range of biological activities, including immunomodulatory, anti-inflammatory and antioxidant properties. Polysaccharides have a variety of applications, including pharmaceuticals, nutraceuticals, and food and beverage products.

Lipids

Lipids are another class of compounds that can be produced by microbial fermentation. We provide fermentation services for the production of lipids, fatty acids with a wide range of biological activities, including anti-inflammatory, antioxidant and anti-cancer properties. Our team of experts can work with you to develop a custom fermentation process that meets your specific needs and requirements. We have state-of-the-art facilities and equipment to ensure that the lipids produced are of the highest quality and purity.

Oligonucleotides and Nucleic Acids

Oligonucleotides and nucleic acids are another class of compounds that can be produced by microbial fermentation. We provide fermentation services for the production of oligonucleotides, which are short chains of nucleotides that are used in a variety of applications, including gene therapy, diagnostics and drug development. Nucleic acids are long chains of nucleotides found in all living organisms and are essential for the storage and transmission of genetic information.

Organic Acids

Organic acids are another class of compounds that can be produced by microbial fermentation. We provide fermentation services for the production of organic acids, which are organic compounds with a wide range of biological activities, including antibacterial, antifungal and antioxidant properties. Whether you want to produce organic acids for research purposes or commercial use, we can help you achieve your goals.

Our Service Advantages

Case Study

Erythromycin is a powerful antibiotic that has shown good results in treating a variety of bacterial infections. However, producing erythromycin through traditional chemical synthesis methods can be costly and environmentally damaging. Microbial fermentation offers a more sustainable and cost-effective alternative for the production of erythromycin. BOC Sciences is a leading microbial fermentation services provider, providing expertise in optimizing fermentation processes to produce bioactive compounds such as erythromycin.

Methods: BOC Sciences will utilize our state-of-the-art fermentation facilities and microbial fermentation expertise to optimize erythromycin production. The fermentation process will involve growing a genetically engineered strain of bacteria capable of producing erythromycin. Fermentation conditions, including temperature, pH, agitation and nutrient composition, will be systematically optimized to maximize erythromycin production. Samples will be collected periodically to monitor bacterial culture growth and erythromycin production.

Results: The optimized fermentation process will lead to a significant increase in erythromycin production compared with traditional methods. The purity and potency of erythromycin produced through microbial fermentation will be confirmed by analytical testing, including high-performance liquid chromatography and mass spectrometry. The scalability of the fermentation process will also be evaluated to determine its feasibility for large-scale production.

FAQ

1. What is the meaning of microbial fermentation?

Microbial fermentation is a process that uses microorganisms such as bacteria, yeast, or fungi to produce a variety of compounds through the fermentation of sugars or other organic compounds. This process has been used for centuries to produce a variety of products, including antibiotics, enzymes, vitamins and amino acids. Today, microbial fermentation is widely used in various industries, including pharmaceuticals, biotechnology, food and beverages, and agriculture.

2. What does microbial fermentation produce?

Microbial fermentation produces a variety of products, including organic acids, alcohols, gases, and other metabolites. Microbial fermentation also produces a variety of other metabolites such as vitamins, amino acids, and enzymes. These metabolites are produced by bacteria and fungi during sugar fermentation and have a variety of industrial and pharmaceutical applications.